An improved direct colorimetric method for the quantitative analysis of urinary hippuric acid as an index of toluene exposure.
نویسندگان
چکیده
An improved direct colorimetric method for determining the concentration of urinary hippuric acid as an index of toluene exposure was described. One tenth ml of urine was diluted with 0.4 ml 0.01 M phosphate buffer H 6.9 and mixed with 0.5 ml pyridine. The mixture was layered on 0.2 ml benzenesulfonyl chloride. The reaction was started by mixing for one min with a mechanical shaker. The colored solution was allowed to stand for 30 min, diluted with 5 ml ethanol, and absorbance measured at 410 nm within 30 min after the dilution. The coefficient of variation of this method was 6% and the recovery 103% when urine contains about 0.2-0.5 mg hippuric acid per ml of urine. The concentration was linear up to 2.0 mg per ml hippuric acid in a specimen.
منابع مشابه
Evaluation of Toluene Exposure in Workers at Industrial Area of Sidoarjo, Indonesia by Measurement of Urinary Hippuric Acid
Background: Toluene is a harmful substance still being used as a solvent and catalyst in many industries that can increase the level of urinary hippuric acid (UHA). This study was designed to examine the level of toluene and UHA among workers of industrial site in seven companies in Sidoarjo, Indonesia and to analyze the risk factors for abnormal UHA. Methods: This study was an analytical cross...
متن کاملComparison of several methods for the measurement of urinary hippuric acid as an index of toluene exposure.
The levels of hippuric acid in the urine of people exposed to toluene vapour were measured by paper chromatography, direct colorimetry, high performance liquid chromatography and gas chromatography. The control was a similar group not exposed to toluene vapour. The values were analyzed statistically, conversion equations calculated, and the propriety of these equation discussed. Since the three...
متن کاملبررسی رابطه پایش محیطی تولوئن و شاخص بیولوژیکی اسید هیپوریک ادرار در افراد شاغل در صنعت کک سازی
Introduction: Toluene is an organic solvent that it is one of the byproducts in the coke industry. Exposure to toluene causes central nervous system dysfunction and others disorders. Many workers are exposed to toluene due to leakage from tracks. Therefore the aim of this study was to determine the levels of exposure through environmental and biological monitoring of toluene Methods: Air tol...
متن کاملبررسی میزان متابولیت های ادراری ترکیبات آلی فرار و عوامل مرتبط با آن در کارگران شاغل در صنعت پتروشیمی در سال1391
Background and purpose: Biological monitoring for workers exposed to solvents includes complementary environmental monitoring which is useful in toxic chemical risk assessments. The aim of this study was environmental and biological monitoring of workers exposed to volatile organic compounds (VOCs) in petrochemical industry. Material and Methods: Air and urine samples were collected from 104 i...
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ورودعنوان ژورنال:
- Acta medica Okayama
دوره 31 4 شماره
صفحات -
تاریخ انتشار 1977